superscript iii first-strand synthesis supermix kit 11752050 (Thermo Fisher)

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Thermo Fisher superscript iii first-strand synthesis supermix kit 11752050
Superscript Iii First Strand Synthesis Supermix Kit 11752050, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/superscript+iii+first-strand+synthesis+supermix+kit/superscript+iii+first+strand+synthesis+supermix+kit+11752050/pmc11898582-212-72-79
Average 90 stars, based on 1 article reviews

superscript iii first-strand synthesis supermix kit 11752050 - by Bioz Stars, 2026-10

90/100 stars

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Article Title: Rescue of Aberrant Splicing Caused by a Novel Complex Deep-intronic ABCA4 Allele
Article Snippet: Subsequently, cDNA was obtained from total RNA with the SuperScript III First-Strand Synthesis SuperMix kit (Invitrogen, Waltham, MA, USA) according to the manufacturer’s instructions.

Article Title: Biosynthesis of the Central Tricyclic Skeleton of Trichothecene Mycotoxins.
Article Snippet: SuperScript III First-Strand Synthesis SuperMix kit was purchased from Thermo Fisher Scientific.

Synthesized:

Article Title: Novel target and cofactor repertoire for the transcriptional regulator JTY_0672 from Mycobacterium bovis BCG
Article Snippet: .. First-strand cDNA was synthesized from 20 ng RNA with SuperScript III First-Strand Synthesis SuperMix kit (Invitrogen), according to the manufacturer’s instructions. .. Quantitative realtime PCR (qRT-PCR) was carried out in a LightCycler480 II (Roche) RT–PCR machine with an iQTM SYBR Green Supermix (Bio-Rad) according to previous study ( ).

Article Title: Preclinical development of TAK-754, a high-performance AAV8-based vector expressing coagulation factor VIII
Article Snippet: Genomic DNA (gDNA) or total RNA was extracted from mouse livers using the DNeasy Blood & Tissue Kit (Qiagen) or the RNeasy mini kit (Qiagen), respectively, following the manufacturer’s protocol for animal tissues. gDNA was quantified using the Qubit dsDNA Broad-Range Kit (Thermo Fisher Scientific). .. Total RNA (1 μg) was treated with DNase (TURBO DNA-free Kit, Thermo Fisher Scientific), and complementary DNA (cDNA) was synthesized using the SuperScript III First-Strand Synthesis SuperMix kit (Thermo Fisher Scientific). ..

Article Title: Preclinical development of TAK-754, a high performance AAV8-based vector expressing coagulation factor VIII
Article Snippet: 448 Detection of hF8 DNA or RNA in mouse organs 449 Genomic DNA (gDNA) or total RNA were extracted from mouse livers using the DNeasy 450 Blood & Tissue Kit (Qiagen) or the RNeasy mini kit (Qiagen), respectively, following the 451 manufacturer's protocol for animal tissues. gDNA was quantified using the Qubit dsDNA 452 Broad-Range Kit (Thermo Fisher Science). .. Total RNA (1μg) was treated with DNase (TURBO 453 DNA-free Kit, Thermo Fisher Scientific) and complementary DNA (cDNA) was synthesized 454 using the SuperScript III First-Strand Synthesis SuperMix kit (Thermo Fisher Scientific). ..

Purification:

Article Title: Chondroitin sulfate regulates proliferation of Drosophila intestinal stem cells.
Article Snippet: Total RNA from 20 5-day old female midguts was extracted using TRIzol (15596026, Thermo Fisher Scientific), treated with RNase-Free DNase I (79524, Qiagen), and purified using a Direct-zol RNA MiniPrep kit (R2050, Zymo Research). .. A SuperScript III First-Strand Synthesis SuperMix kit (18080–400, Thermo Fisher Scientific) was used to synthesize the purified RNA to cDNA. .. Using LightCycler 480 SYBR Green I Master mix (04707516001, Roche Diagnostics) in a LightCycler 480 Instrument II (Roche, Basel, Switzerland), qPCR assays were run on triplicate for each of the four independent biological replicates.

Reverse Transcription:

Article Title: Myeloid-derived suppressor cell inhibits T-cell-based defense against Klebsiella pneumoniae infection via IDO1 production.
Article Snippet: RNA extraction and real-time quantitative PCR analysis Total RNA was extracted from lung samples, PMN-MDSCs, T cells, MDSCs and Tregs in TRIzol reagent (#15596026, Thermo Fisher Scientific), followed by chloroform extraction and isopropanol precipitation. .. The extracted RNA was reverse-transcribed into cDNA by using a SuperScript III First-Strand Synthesis SuperMix kit (#11752050, Thermo Fisher Scientific). ..

cDNA Synthesis:

Article Title: Sheep serve as amplifying hosts of Japanese encephalitis virus, increasing the risk of human infection
Article Snippet: Viral RNA was extracted from the passage 5 stock of the JEV SH2201 strain using Trizol reagent (Thermo Fisher Scientific, USA, catalog no.15596018CN) in accordance with the manufacturer’s instructions for whole-genome sequencing. .. The cDNA synthesis was performed using the SuperScript III First-Strand Synthesis SuperMix kit in according with the manufacturer’s protocol (Thermo Fisher Scientific, USA, catalog no.18080400). .. The resulting cDNA was subsequently subjected to Illumina pair-end sequencing (Tgene Biotech, Shanghai, China).

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